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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
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Catalogue No: abx005568
Price:
US$290.00
(Size: 100 µl)
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GAPDH Antibody is a Rabbit Polyclonal antibody against GAPDH. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transport, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PACR and also loading control in electrophoresis and Western blotting. This antibody is a rabbit polyclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Research Articles on Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
| Target | Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | WB, IHC, IF/ICC, IP |
| Host | Rabbit |
| Recommended dilutions | WB: 1/10000 - 1/30000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P04406 (UniProt, ExPASy) |
| UniProt Secondary AC | E7EUT4, P00354, Q53X65 |
| UniProt Entry Name | G3P_HUMAN |
| GeneID | 2597 |
| NCBI Accession | NP_002037.2 |
| KEGG | hsa:2597 |
| String | 9606.ENSP00000229239 |
| Molecular Weight | Calculated MW: 36 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |