Guanylyl Cyclase-Activating Protein 1 (GUCA1A) Antibody

Guanylate cyclase-activating protein is a l Ca (2+) binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca (2+) binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

Target	Guanylyl Cyclase-Activating Protein 1 (GUCA1A)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 160-191 amino acids from the C-terminal region of human GCAP1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P43080 (UniProt, ExPASy)
UniProt Secondary AC	B3KWT4, Q7Z6T1, Q9NU14
UniProt Entry Name	GUC1A_HUMAN
KEGG	hsa:2978
String	9606.ENSP00000377784
Molecular Weight	Calculated MW: 22.9 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Cow GUCA1A.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Guanylyl Cyclase-Activating Protein 1 (GUCA1A)

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