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Target | Staphylococcal Protein A |
Origin | Bacteria |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 90% |
Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P38507 (UniProt, ExPASy) |
UniProt Entry Name | SPA_STAAU |
Molecular Weight | Calculated MW: 35.46 kDa Observed MW (SDS-PAGE): 35 kDa |
Sequence Fragment | Thr31-Pro334 |
Sequence | TPAANAAQHD EAQQNAFYQV LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF NKDQQSAFYE ILNMPNLNEE QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLAE AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKP |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Not tested |
Concentration | Prior to lyophilization: 200 µg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |