+44 (0) 1223 755950
+1 832 327 7413
Green Fluorescent Protein (GFP) Antibody
REQUEST MORE INFO
Catalogue No: abx005585
Price:
US$290.00
(Size: 100 µl)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
GFP-Tag Antibody is a Mouse Monoclonal antibody against GFP-Tag. Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
Research Articles on Green Fluorescent Protein (GFP)
| Target | Green Fluorescent Protein (GFP) |
| Clonality | Monoclonal |
| Reactivity | General (All species) |
| Tested Applications | WB, IP |
| Host | Mouse |
| Recommended dilutions | WB: 1/10000 - 1/40000, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 to the N-terminus of GFP protein. |
| Isotype | IgG1 Kappa |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight | Calculated MW: 27 kDa Observed MW: 27 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |