Glucokinase (GCK) Assay Kit Assay Kit is an assay kit for detection and quantification of Glucokinase concentrations in tissue homogenates, cell lysates and cell culture supernatants using a microplate reader. Glucokinase (GCK) is a key enzyme in the glucose metabolism pathway. It participates in the first step of glycolysis, catalyzes glucose phosphorylation, promotes insulin secretion and glucose metabolism, and effectively controls blood glucose balance. GCK, also known as hexokinase IV, is mainly expressed in hepatocytes and islet beta-cells, and has a stronger affinity for glucose than other hexokinases. GCK catalyzes the substrate reaction to produce NADPH, which reacts with the chromogenic agent. The absorbance values of the standard tube and the sample tube are measured respectively, and the Glucokinase content in the sample can be calculated.

Target	Glucokinase (GCK)
Reactivity	General (All species)
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. Avoid exposure to light.
Validity	The validity for this kit is 12 months.
Test Range	2.62 U/L - 42.80 U/L
Sensitivity	2.62 U/L
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Tissue homogenates, cell lysates and cell culture supernatants.
Availability	Shipped within 7-12 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Research Articles on Glucokinase (GCK)

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