Glutathione Peroxidase (GPx) Assay Kit can be used to detect Glutathione Peroxidase (GPx) in serum, plasma, tissue homogenates, cell lysates and cell culture supernatants using a microplate reader. Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. The concentration of GSH can be determined by measuring the absorbance at 412 nm.

Target	Glutathione Peroxidase (GPx)
Reactivity	General (All species)
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 12 months.
Test Range	17.17 U - 518.32 U
Sensitivity	17.17 U
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Serum, plasma, tissue homogenates, cell lysates and cell culture supernatants.
CAS Number	9013-66-5
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Glutathione Peroxidase (GPx)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue