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Target | Endoplasmic Reticulum Chaperone BiP (HSPA5) |
Origin | Human |
Expression | Recombinant |
Tested Applications | ELISA, WB, SDS-PAGE |
Host | E. coli |
Purity | > 90% |
Purification | Purified by affinity chromatography. |
Storage | Store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P11021 (UniProt, ExPASy) |
UniProt Secondary AC | B0QZ61, Q2EF78, Q9NPF1, Q9UK02 |
UniProt Entry Name | BIP_HUMAN |
Gene Symbol | HSPA5 |
GeneID | 3309 |
NCBI Accession | NM_005347 |
KEGG | hsa:3309 |
String | 9606.ENSP00000324173 |
Molecular Weight | 78 kDa |
Sequence Fragment | Full Length |
Sequence | MKLSLVAAML LLLSAARAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV EIIANDQGNR ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG RTWNDPSVQQ DIKFLPFKVV EKKTKPYIQV DIGGGQTKTF APEEISAMVL TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVMRIIN EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG VFEVVATNGD THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS SQHQARIEIE SFYEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD LKKSDIDEIV LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV QAGVLSGDQD TGDLVLLDVC PLTLGIETVG GVMTKLIPRN TVVPTKKSQI FSTASDNQPT VTIKVYEGER PLTKDNHLLG TFDLTGIPPA PRGVPQIEVT FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER MVNDAEKFAE EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSSED KETMEKAVEE KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSAGP PPTGEEDTAE KDEL |
Tag | His tag |
Buffer | 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 10% glycerol. |
Activity | Active |
Biological Activity | ATPase active: liberates 2.3 µM phosphate /hr/µg protein in 200 µl reaction volume at 37 °C, pH 8, in the presence of 20 µl of 1 mM ATP (Malachite Green assay). |
Concentration | 1.2 mg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |