H. pylori Ferritin Protein

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Catalogue No: abx620125
Price: US$1,740.00
(Size: 1 mg)
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SDS-PAGE analysis, with Coomassie blue staining, of expression tests to determine induction strategy, temperature and time for induction, and E. coli strain to use for protein production. The negative control is marked Ø. Briefly, bacteria were obtained by incubation at 37 °C. IPTG induction was carried out at 16 °C for 16 h or 37 °C for 4 h. Bacterial cells were collected by centrifugation and lysed in a buffer containing PBS, pH 7.5, 10% glycerol. After centrifugation, the soluble supernatnant fraction was collected (native protein extraction - NPE). The insoluble fraction was collected and solubilized with a denaturing buffer (8 M urea). After centrifugation, the supernatant was collected (denatured protein extraction - DPE). Based on the expression tests, the optimal conditions were: BL21(DE3) strain, IPTG induction at 37 °C for 4 h, DPE.
H. pylori Ferritin Protein H. pylori Ferritin Protein

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Documents

Datasheet SDS
Helicobacter pylori Ferritin Protein is a recombinant protein expressed in E. coli.

Target Ferritin
Origin Bacteria
Expression Recombinant
Tested Applications SDS-PAGE
Host E. coli
Form Lyophilized
Purity > 90%
Reconstitution Reconstitute in ddH2O. Do not vortex.
Storage Store at -20 °C. Avoid repeated freeze/thaw cycles.
NCBI Accession WP_000949190.1
Sequence Fragment Met1-Ser167
Sequence MGSSHHHHHHSSG LVPRGSH MLSKDIIKLL NEQVNKEMNS SNLYMSMSSW CYTHSLDGAG LFLFDHAAEE YEHAKKLIIF LNENNVPVQL TSISAPEHKF EGLTQIFQKA YEHEQHISES INNIVDHAIK SKDHATFNFL QWYVAEQHEE EVLFKDILDK IELIGNENHG LYLADQYVKG IAKSRKS
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.5.
Activity Not tested
Concentration Prior to lyophilization: 0.40 mg/ml
Availability Shipped within 1-2 months.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Ferritin


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