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Histone H2A.X (H2AFX) Antibody
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Catalogue No: abx034840
Price:
US$326.25
(Size: 80 µl)
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Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Research Articles on Histone H2A.X (H2AFX)
| Target | Histone H2A.X (H2AFX) |
| Clonality | Monoclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Mouse |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/25. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P16104 (UniProt, ExPASy) |
| UniProt Secondary AC | Q4ZGJ7, Q6IAS5 |
| UniProt Entry Name | H2AX_HUMAN |
| NCBI Accession | NP_002096.1 |
| KEGG | hsa:3014 |
| String | 9606.ENSP00000434024 |
| Molecular Weight | Calculated MW: 15.1 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse and Zebrafish H2AX. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |