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Trifunctional Enzyme Subunit Alpha, Mitochondrial (HADHA) Antibody
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Catalogue No: abx004088
Price:
US$232.00
(Size: 20 µl)
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![Western blot analysis of lysates from K-562 cells, using [KO Validated] HADHA Antibody at 1/5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.](https://www.abbexa.com/image/cache/data/products/18B/abx004088_1-500x500.jpg "Trifunctional Enzyme Subunit Alpha, Mitochondrial (HADHA) Antibody")
HADHA Antibody is a Rabbit Polyclonal antibody against HADHA. This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Research Articles on Trifunctional Enzyme Subunit Alpha, Mitochondrial (HADHA)
| Target | Trifunctional Enzyme Subunit Alpha, Mitochondrial (HADHA) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF/ICC, IP |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/2000 - 1/6000, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P40939 (UniProt, ExPASy) |
| UniProt Secondary AC | B2R7L4, B4DYP2, Q16679, Q53T69, Q53TA2, Q96GT7, Q9UQC5 |
| UniProt Entry Name | ECHA_HUMAN |
| Gene Symbol | HADHA |
| GeneID | 3030 |
| NCBI Accession | NP_000173.2 |
| KEGG | hsa:3030 |
| String | 9606.ENSP00000370023 |
| Molecular Weight | Calculated MW: 83 kDa Observed MW: 78 kDa |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |