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Target | HCV Core/NS3/NS4 |
Origin | Virus |
Expression | Recombinant |
Tested Applications | ELISA |
Host | E. coli |
Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
Form | Lyophilized |
Purity | > 95% (SDS-PAGE) |
Purification | Purified by Q-Sepharose FF, S-Sepharose FF and Sephadex G-50. |
Reconstitution | Reconstitute in distilled water. The distilled water must not contain potassium ions because SDS and potassium ions generate flocculent precipitate. Tris can be removed by dialysis. It is recommended to add 0.02% SDS to the buffer used for dialysis. SDS is required for recombinant antigens to dissolve, but the content is very low and will not affect avidin connection. |
Storage | Store at -20 °C in lyophilized form for long-term storage. After reconstitution, store at 2-8 °C for up to 3 days. Avoid repeated freeze/thaw cycles. |
Sequence Fragment | Contains three antigens expressed in E. coli in a chimera:
|
Buffer | Prior to lyophilization: 0.01 M Tris, pH 8.0, 0.02% SDS. Does not contain preservatives. |
Concentration | Prior to lyophilization: 41.8 mg/ml |
Availability | Shipped within 5-20 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Directions for use | Centrifuge before opening to ensure complete recovery of vial contents. |