Hematopoietic Progenitor Cell Antigen CD34 (CD34) Antibody

REQUEST MORE INFO
Catalogue No: abx227125
Price: US$319.00
(Size: 40 µl)

Orders to United States ship from United States

Reviews: 0 Publications and Reviews
(0 Publications and Reviews)
IHC-P analysis showing focal CD34 positivity in the gastrointestinal stromal tumor (GIST) of small intestine (4 µm section).
Hematopoietic Progenitor Cell Antigen CD34 (CD34) Antibody Hematopoietic Progenitor Cell Antigen CD34 (CD34) Antibody

Click on the image to see the image legend

Available Options

* Size:





Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Hematopoietic Progenitor Cell Antigen CD34 (CD34) Antibody is a Rabbit Monoclonal antibody for the detection of CD34.

Target Hematopoietic Progenitor Cell Antigen CD34 (CD34)
Clonality Monoclonal
Clone G759
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the N-terminal region of human CD34.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P28906 (UniProt, ExPASy)
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20.
  3. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  4. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  5. Rinse twice in distilled water, 5 minutes each.
  6. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  7. Wash twice in distilled water, 5 minutes each.
  8. Antigen retrieval: immerse the slide in Citrate buffer, pH 6.0, and incubate in a water bath for 25 minutes at 95-97 °C.
  9. Remove the slide from the water bath and allow to stand at room temperature (in Citrate buffer, pH 6.0) for 15 minutes.
  10. Rinse twice in distilled water, 5 minutes each.
  11. Wash twice in Wash Buffer, 5 minutes each.
  12. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
  13. Wash 3 times with Wash Buffer, 5 minutes each.
  14. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  15. Wash 3 times in Wash Buffer, 5 minutes each.
  16. Apply the DAB chromagen for 1-3 minutes.
  17. Wash twice in water, 5 minutes each.
  18. Stain in hematoxylin for 5 minutes.
  19. Wash 3 times in water, 2 minutes each.
  20. Mount the slide for observation.
Research Articles on Hematopoietic Progenitor Cell Antigen CD34 (CD34)
Tags:
(Click to show)