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HEPES Solution
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Catalogue No: abx295003
Price:
US$58.00
(Size: 100 ml)
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Datasheet SDS
HEPES is a buffer that is widely used in biological research, particularly in cell and tissue culture. It is zwitterionic at physiological pH and has negligible metal ion binding, and therefore has little to no effect on the vast majority of biochemical reactions. It has a pKa of 7.5 (at 25 °C) and is effective between pH 6.8-8.2.
Research Articles on HEPES
| Target | HEPES |
| Recommended dilutions | Working concentration: 10-50 mM, Cell Culture: 10 mM. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
| Purification | Sterilized by 0.1 µm filtration. |
| Storage | Store between 2-8 °C for short-term storage. For long-term storage, store at -20 °C. Avoid exposure to light. |
| Validity | Up to 24 months. |
| Buffer | 238.3 g/L HEPES in ultrapure water, pH 7.2-7.4. |
| CAS Number | 7365-45-9 |
| Endotoxin Level | < 1 EU/ml |
| Concentration | 1 M |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. |
| Directions for use | This product may be used directly without dilution. Use aseptic techniques to avoid contamination. If HEPES is exposed to light for more than 3 hours, it may undergo oxidation to produce hydrogen peroxide, which has a toxic effect on cells. |