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Hepatocyte Growth Factor (HGF) Antibody
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Catalogue No: abx001106
Price:
US$203.00
(Size: 20 µl)
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HGF Antibody is a Rabbit Polyclonal antibody against HGF. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Research Articles on Hepatocyte Growth Factor (HGF)
| Target | Hepatocyte Growth Factor (HGF) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 106-160 of human HGF. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P14210 (UniProt, ExPASy) |
| UniProt Entry Name | HGF_HUMAN |
| Gene Symbol | HGF |
| GeneID | 3082 |
| NCBI Accession | NP_000592.3 |
| KEGG | hsa:3082 |
| String | 9606.ENSP00000222390 |
| Molecular Weight | Calculated MW: 83 kDa Observed MW: 62 kDa. Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Concentration | 0.17 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |