HindIII Enzyme

HindIII is expressed and purified from E. coli that carries the recombinant HindIII gene. The recognition site is A^AGCTT. The reaction is conducted for 5-10 minutes at 37 °C, and heat-inactivated at 65 °C for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Target	HindIII
Epitope Region	Recognition site: 5’...A↓AGCTT...3’ 3’...TTCGA↑A...5’
Form	Liquid
Storage	Store at -20°C. Stable for 12 months from date of receipt.
Molecular Weight	35.8 kDa
Buffer	HindIII storage buffer: 20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 µg/ml BSA, 50% Glycerol. 10x buffer: 500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA.
Concentration	20,000 units/ml
Availability	Shipped within 10-20 working days.
Note	This product is for research use only. This product is shipped with dry ice. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on HindIII

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