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His Tag Antibody
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Catalogue No: abx005576
Price:
US$145.00
(Size: 20 µl)
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His Tag Antibody is a Mouse Monoclonal against His Tag. A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Research Articles on His Tag
| Target | His Tag |
| Clonality | Monoclonal |
| Reactivity | General (All species) |
| Tested Applications | ELISA, WB, IF/ICC, IP |
| Host | Mouse |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/5000 - 1/20000, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide corresponding to His tag. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight | Observed MW: 27 kDa, 35 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | 5.26 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |