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Histone H4 (H4) ELISA Kit
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Catalogue No: abx153543
Price:
US$812.00
(Size: 96 tests)
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Histone H4 (H4) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human, Mouse, Rat Histone H4 (H4) concentrations in tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This assay has high sensitivity and excellent specificity for detection of Histone H4 (H4)
No significant cross-reactivity or interference between Histone H4 (H4) and analogues was observed.
Research Articles on Histone H4 (H4)
No significant cross-reactivity or interference between Histone H4 (H4) and analogues was observed.
| Introduction | Histone H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosome of the 'beads on a string' organization. Histone proteins are highly post-translationally modified. Covalently bonded modifications include acetylation and methylation of the N-terminal tails. These modifications may alter expression of genes located on DNA associated with its parent histone octamer. Histone H4 is an important protein in the structure and function of chromatin, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. |
| Target | Histone H4 (H4) |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request. |
| Stability | The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout. |
| Test Range | 78 pg/ml - 5000 pg/ml |
| Standard Form | Lyophilized |
| Detection Method | Colorimetric |
| Assay Type | Sandwich |
| Assay Data | Quantitative |
| Sample Type | Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
| Availability | Shipped within 5-12 working days. |
| Note | This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
| Standard | 40 pg/ml |