H+K+-ATPase Assay Kit is an assay kit for the detection and quantification of H+K+-ATPase activity in animal tissue homogenates and cell lysates using a spectrophotometer. ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. By determining the concentration of molybdenum blue, the concentration of inorganic phosphorus can be calculated.

Target	H+K+-ATPase
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 12 months.
Detection Method	Colorimetric
Sample Type	Animal tissue homogenates and cell lysates.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on H+K+-ATPase

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