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Target | HLA-Class I |
Clonality | Monoclonal |
Clone | D117 |
Reactivity | Human |
Tested Applications | WB, FCM, IP |
Host | Mouse |
Recommended dilutions | FCM: 1-5 μg/ml. WB validated in non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | PHA-activated peripheral blood lymphocytes. |
Isotype | IgG1 |
Purity | > 95% (SDS-PAGE) |
Purification | Purified by Protein A affinity chromatography. |
Storage | Store at 2-8°C. Do not freeze. |
UniProt Primary AC | P04439 (UniProt, ExPASy) |
UniProt Secondary AC | B1PKZ3, O02939, O02954, O02955, O02963, O19509, O19546, O19598, O19605, O19606, O19619, O19647, O19673, O19687, O19695, O19756, O19794, O19795, O43906, O43907, O46874, O62921, O62924, O77937, O77938, O77964, O78073, O78171, O98009, O98010, O98011, O98137, P01891, P01892, P05534, P06338, P10313, P10314, P10315, P10316, P13746, P16188, P16189, P16190, P18462, P30443, P30444, P30445, P30446, P30447, P30448, P30449, P30450, P30451, P30452, P30453, P30454, P30455, P30456, P30457, P30458, P30459, P30512, P30514, P79505, P79562, P79563, Q09160, Q29680, Q29747, Q29835, Q29837, Q29838, Q29899, Q29908, Q29909, Q29910, Q30208, Q31623, Q5S3G1, Q65A82, Q8MHM1, Q8MHN9, Q95352, Q95355, Q95362, Q95377, Q95380, Q95IZ5, Q9BCN0, Q9BD15, Q9BD19, Q9GJE6, Q9GJE7, Q9GJE8, Q9MW42, Q9MY89, Q9MYA3, Q9MYA5, Q9MYC4, Q9MYE6, Q9MYE9, Q9MYG4, Q9MYG5, Q9MYI5, Q9TP25, Q9TPQ3, Q9TPR8, Q9TPX8, Q9TPX9, Q9TPY0, Q9TQ24, Q9TQE8, Q9TQE9, Q9TQF1, Q9TQF5, Q9TQF8, Q9TQF9, Q9TQG0, Q9TQG5, Q9TQG7, Q9TQH5, Q9TQI3, Q9TQK5, Q9TQM6, Q9TQN5, Q9TQP5, Q9TQP6, Q9TQP7, Q9UIN1, Q9UIN2, Q9UIP7, Q9UQU3, Q9UQU6, Q9UQU7 |
GeneID | 3105 |
Buffer | PBS solution with 15 mM sodium azide. |
Specificity | The antibody reacts with all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32. |
Concentration | 1 mg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. |