HRP Antibody

Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots.They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates.The speed and sensitivity of these methods are unequalled by traditional alternatives.Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies.HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal.HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.

Target	HRP
Clonality	Monoclonal
Reactivity	General (All species)
Tested Applications	ELISA
Host	Mouse
Recommended dilutions	ELISA: 1/10000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of HRP expressed in E. coli.
Isotype	IgG1
Form	Liquid
Purification	Unpurified ascites.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Buffer	Ascitic fluid containing 0.03% sodium azide.
Concentration	Not determined.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on HRP

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