Human 5-Hydroxytryptamine Receptor 1A / 5-HT1A (HTR1A) Protein

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Catalogue No: abx652344
Price: US$246.50
(Size: 10 µg)

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Human 5-Hydroxytryptamine Receptor 1A / 5-HT1A (HTR1A) Protein Human 5-Hydroxytryptamine Receptor 1A / 5-HT1A (HTR1A) Protein

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Datasheet SDS
Human 5-Hydroxytryptamine Receptor 1A (HTR1A) Protein is a Recombinant Human protein expressed in E. coli.

Target 5-Hydroxytryptamine Receptor 1A / 5-HT1A (HTR1A)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 80%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P08908 (UniProt, ExPASy)
UniProt Entry Name 5HT1A_HUMAN
Molecular Weight Calculated MW: 66.5 kDa
Observed MW (SDS-PAGE): 70 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Met1-Gln422
Sequence MDVLSPGQGN NTTSPPAPFE TGGNTTGISD VTVSYQVITS LLLGTLIFCA VLGNACVVAA IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN KWTLGQVTCD LFIALDVLCC TSSILHLCAI ALDRYWAITD PIDYVNKRTP RRAAALISLT WLIGFLISIP PMLGWRTPED RSDPDACTIS KDHGYTIYST FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKTGADT RHGASPAPQP KKSVNGESGS RNWRLGVESK AGGALCANGA VRQGDDGAAL EVIEVHRVGN SKEHLPLPSE AGPTPCAPAS FERKNERNAE AKRKMALARE RKTVKTLGII MGTFILCWLP FFIVALVLPF CESSCHMPTL LGAIINWLGY SNSLLNPVIY AYFNKDFQNA FKKIIKCKFC RQ
Tag N-terminal His tag and TrxA tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 80 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on 5-Hydroxytryptamine Receptor 1A / 5-HT1A (HTR1A)