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Human Aspartate Aminotransferase (AST) Protein (Active)
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Catalogue No: abx655961
Price:
US$725.00
(Size: 50 µg)
Click on the image to see the image legend
Human Aspartate Aminotransferase (AST) Protein (Active) is an active recombinant Human protein produced in a Eukaryotic expression system (293F cell).
Research Articles on Aspartate Aminotransferase (AST)
| Target | Aspartate Aminotransferase (AST) | ||||||||
| Origin | Human | ||||||||
| Expression | Recombinant | ||||||||
| Tested Applications | WB, SDS-PAGE | ||||||||
| Host | 293F cell | ||||||||
| Conjugation | Unconjugated | ||||||||
| Form | Lyophilized | ||||||||
| Purity | > 95% | ||||||||
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.6. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.6, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. | ||||||||
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. | ||||||||
| UniProt Primary AC | P17405 (UniProt, ExPASy) | ||||||||
| UniProt Secondary AC | A8K8M3, E9PKS3, P17406, Q13811, Q16837, Q16841 | ||||||||
| UniProt Entry Name | ASM_HUMAN | ||||||||
| Gene Symbol | SMPD1 | ||||||||
| GeneID | 6609 | ||||||||
| OMIM | 607608 | ||||||||
| HGNC | 11120 | ||||||||
| KEGG | hsa:6609 | ||||||||
| Ensembl | ENSG00000166311 | ||||||||
| String | 9606.ENSP00000340409 | ||||||||
| Molecular Weight | Calculated MW: 64.9 kDa Observed MW (SDS-PAGE): 75 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
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| Sequence Fragment | His62-Pro628 | ||||||||
| Sequence | HPLSPQGHP ARLHRIVPRL RDVFGWGNLT CPICKGLFTA INLGLKKEPN VARVGSVAIK LCNLLKIAPP AVCQSIVHLF EDDMVEVWRR SVLSPSEACG LLLGSTCGHW DIFSSWNISL PTVPKPPPKP PSPPAPGAPV SRILFLTDLH WDHDYLEGTD PDCADPLCCR RGSGLPPASR PGAGYWGEYS KCDLPLRTLE SLLSGLGPAG PFDMVYWTGD IPAHDVWHQT RQDQLRALTT VTALVRKFLG PVPVYPAVGN HESTPVNSFP PPFIEGNHSS RWLYEAMAKA WEPWLPAEAL RTLRIGGFYA LSPYPGLRLI SLNMNFCSRE NFWLLINSTD PAGQLQWLVG ELQAAEDRGD KVHIIGHIPP GHCLKSWSWN YYRIVARYEN TLAAQFFGHT HVDEFEVFYD EETLSRPLAV AFLAPSATTY IGLNPGYRVY QIDGNYSGSS HVVLDHETYI LNLTQANIPG AIPHWQLLYR ARETYGLPNT LPTAWHNLVY RMRGDMQLFQ TFWFLYHKGH PPSEPCGTPC RLATLCAQLS ARADSPALCR HLMPDGSLPE AQSLWPRP | ||||||||
| Tag | N-terminal His tag | ||||||||
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose. | ||||||||
| Activity | Active | ||||||||
| Biological Activity | Specific activity: 4.9 U/mg In this assay, an amino group is exchanged from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The oxaloacetic acid can be decomposed into pyruvate and carbon dioxide in the presence of phenylamine citrate. The activity of aspartate transaminase can be measured by calculating the concentration of the pyruvate. Blank, sample and standard tubes were prepared and labelled. 10 µl of different concentrations of recombinant AST were added to respective sample tubes. 10 µl of phosphate buffer was added to the blank tube in place of AST. The standard curve was prepared by 2-fold dilution of 2 µM pyruvate with phosphate buffer. 50 µl of substrate containing 2 mM 2-Ketoglutaric acid, 0.1 M L-aspartic acid, 0.2 M phosphate buffer, pH 7.4, was added to all vials. All vials were incubated at 37 °C for 1 hour. 10 µl phenylamine citrate and 50 µl 2,4-dinitrophenylhydrazine were added to all vials. The vials were incubated at 37 °C for 20 min. 500 µl of 0.4 M NaOH was added to the blank and sample tubes to stop the reaction, and then the OD value was recorded at 520 nm.One unit of AST Activity (U) is the amount of enzyme that generates 1 μmol of pyruvate per minute (pH 7.4, 37 °C). AST Activity (U/mg) = OD × d t × P where:
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| Concentration | Prior to lyophilization: 200 µg/ml | ||||||||
| Availability | Shipped within 5-7 working days. | ||||||||
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
