Human Adenosine Deaminase 2 (ADA2) ELISA Kit

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Catalogue No: abx055599
Price: US$652.50
(Size: 96 tests)
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Graph showing standard OD data for Human Adenosine Deaminase 2 (ADA2)
Human Adenosine Deaminase 2 (ADA2) ELISA Kit

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Datasheet SDS Download Manual
Human Adenosine Deaminase 2 (ADA2) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Adenosine Deaminase 2 (ADA2) concentrations in serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Introduction Adenosine Deaminase 2 (ADA2, also known as Cat eye syndrome critical region protein 1, CECR1) is an enzyme that deaminates adenosine to inosine, using zinc as its catalytic core. This enzyme’s function is poorly described; it is thought to be involved in cell proliferation. Overexpression of this protein can occur in cancers, or as a result of a duplication of the q arm of chromosome 22, resulting in Cat Eye Syndrome; symptoms include anal atresia, iris coloboma, musculoskeletal issues, and kidney malformation. Mutations in ADA2 are associated with autism spectrum disorders.
Target Adenosine Deaminase 2 (ADA2)
Reactivity Human
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
UniProt Primary AC Q9NZK5 (UniProt, ExPASy)
UniProt Secondary AC A8K9H4, Q6ICF1, Q86UB6, Q8NCJ2, Q96K41
UniProt Entry Name ADA2_HUMAN
Gene Symbol ADA2
GeneID 51816
OMIM 182410
HGNC 1839
String 9606.ENSP00000382731
Test Range 62.5 pg/ml - 4000 pg/ml
Sensitivity 37.5 pg/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Sandwich
Assay Data Quantitative
Sample Type Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Kit Components The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • Pre-coated 96-Well Microplate
  • Standard
  • Standard Diluent Buffer
  • Wash Buffer
  • Detection Reagent A
  • Detection Reagent B
  • Diluent A
  • Diluent B
  • TMB Substrate
  • Stop Solution
  • Plate Sealer
Material Required But Not Provided
  • 37°C incubator
  • Multi and single channel pipettes and sterile pipette tips
  • Squirt bottle or automated microplate washer
  • 1.5 ml tubes
  • Distilled water
  • Absorbent filter papers
  • 100 ml and 1 liter graduated cylinders
  • Microplate reader (wavelength: 450 nm)
  • ELISA Shaker
Assay Procedure This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
  • 1) Set standard, test samples and control wells.
  • 2) Aliquot 100 µl of diluted standard into the standard wells.
  • 3) Aliquot 100 µl of Standard Diluent buffer into control (zero) well.
  • 4) Aliquot 100 µl of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
  • 5) Aliquot 100 µl of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
  • 6) Wash 3 times.
  • 7) Aliquot 100 µl of Detection Reagent B to each well. Incubate for 90 mins at 37 °C.
  • 8) Wash 5 times.
  • 9) Aliquot 90 µl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
  • 10) Aliquot 50 µl of Stop Solution.
  • 11) Measure the OD at 450 nm.
Assay Precision Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Adenosine Deaminase 2 (ADA2) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Adenosine Deaminase 2 (ADA2) were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) × 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%
Availability Shipped within 5-7 working days.
Note This product is for research use only.

The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.

Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Plate coated with Antibody
Research Articles on Adenosine Deaminase 2 (ADA2)


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