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Target | Epidermal Growth Factor Receptor (EGFR) |
Origin | Human |
Expression | Recombinant |
Tested Applications | SDS-PAGE |
Host | Mammalian cells |
Form | Lyophilized |
Purity | > 90% (SDS-PAGE) |
Purification | 0.2 µm filtered prior to lyophilization. |
Storage | Store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
Validity | 6 months in liquid form, or 12 months in lyophilized form. |
UniProt Primary AC | P00533 (UniProt, ExPASy) |
UniProt Entry Name | EGFR_HUMAN |
Gene Symbol | EGFR |
KEGG | hsa:1956 |
String | 9606.ENSP00000275493 |
Molecular Weight | 73.6 kDa |
Sequence Fragment | 25-645 AA. |
Sequence | LEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCN LLEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPS |
Tag | N-terminal His tag and C-terminal Myc tag |
Buffer | Prior to lyophilization: 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. |
Activity | Active |
Biological Activity | Biological activity was measured by binding ability in functional ELISA. Immobilized EGFR at 1 µg/ml binds human EGFR antibody, the EC50 of human EGFR protein is 2.9-3.6 ng/ml. |
Endotoxin Level | < 1.0 EU/ug as determined by LAL method. |
Availability | Shipped within 5-15 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |