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Human Fatty Acid Binding Protein 4, Adipocyte (FABP4) Protein (Active)
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Catalogue No: abx655618
Price:
US$536.50
(Size: 50 µg)
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Human Fatty Acid Binding Protein 4, Adipocyte (FABP4) Protein (Active) is an Active Recombinant Human protein expressed in 293F cells.
Research Articles on Fatty Acid Binding Protein 4, Adipocyte (FABP4)
| Target | Fatty Acid Binding Protein 4, Adipocyte (FABP4) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cell |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P15090 (UniProt, ExPASy) |
| UniProt Secondary AC | Q6IBA1 |
| UniProt Entry Name | FABP4_HUMAN |
| KEGG | hsa:2167 |
| String | 9606.ENSP00000256104 |
| Molecular Weight | Calculated MW: 16.2 kDa Observed MW (SDS-PAGE): 16 kDa |
| Sequence Fragment | Cys2-Ala132 |
| Sequence | CDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Fatty Acid Binding Protein 4, Adipocyte (FABP4) encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may be involved in fatty acid uptake, transport, and metabolism. Actin Beta (ACTB) has been identified as an interactor of FABP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP4 and recombinant human ACTB. Briefly, FABP4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTB-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-FABP4 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of FABP4 and ACTB is shown in Figure 4. |
| Endotoxin Level | < 1.0 EU/µg protein (LAL method) |
| Concentration | Prior to lyophilization: 100 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |