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Target | Fibronectin (FN1) |
Origin | Human |
Expression | Recombinant |
Tested Applications | SDS-PAGE |
Host | E. coli |
Recommended dilutions | Cell attachment and spreading in cell culture: 1-5 µg/cm2, Cell media: 0.5-50 µg/ml. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 95% (RP-HPLC and SDS-PAGE) |
Purification | Purified by proprietary chromatographic techniques. 0.2 µm filtered prior to lyophilization. |
Reconstitution | Reconstitute in sterile 18 MΩ · cm water to a concentration of at least 0.1 mg/ml. This stock solution can be further diluted if required. |
Storage | Store lyophilized at -20 °C. Upon reconstitution, store at 4 °C for up to 1 week, or aliquot and store at or below -20 °C for long-term storage. Avoid repeated freeze/thaw cycles. |
Molecular Weight | 62.6 kDa |
Sequence Fragment | 574 AA |
Buffer | Prior to lyophilization: 20 mM Tris HCl, pH 8.0, containing 150 mM NaCl, 5% Trehalose and 0.02% Tween-20. |
Biological Activity | Determined by the ability to support cell attachment and spreading when used as a substrate in cell culture. |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |