Human G Protein Coupled Receptor 35 (GPR35) Protein

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Catalogue No: abx653488
Price: US$232.00
(Size: 10 µg)

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Datasheet SDS
Human G Protein Coupled Receptor 35 (GPR35) Protein is a Recombinant Human protein expressed in E. coli.

This protein is the immunogen for the following antibodies: abx172494

Target G Protein Coupled Receptor 35 (GPR35)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q9HC97 (UniProt, ExPASy)
UniProt Secondary AC J3KR30, O43495, Q17R58, Q4VBN5, Q4ZFV2, Q6FHI8, Q86UH4
UniProt Entry Name GPR35_HUMAN
Gene Symbol GPR35
GeneID 2859
OMIM 602646
HGNC 4492
Ensembl ENSG00000178623
String 9606.ENSP00000411788
Molecular Weight Calculated MW: 37.7 kDa
Observed MW (SDS-PAGE): 41 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Arg240-Ala309
Sequence R LAVGWNACAL LETIRRALYI TSKLSDANCC LDAICYYYMA KEFQEASALA VAPSAKAHKS QDSLCVTLA
Tag N-terminal His tag and GST tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 500 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on G Protein Coupled Receptor 35 (GPR35)