Human Heparanase (HPA) Protein (Active)

Human Heparanase (HPA) Protein (Active) is a recombinant active Human protein.

Target	Heparanase (HPA)
Origin	Human
Expression	Recombinant
Tested Applications	ELISA, WB, IP, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 97%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9Y251 (UniProt, ExPASy)
UniProt Secondary AC	A9JIG7, C7F7I3, C7F7I4, E9PCA9, E9PGR1, Q53GE5, Q9UL39
UniProt Entry Name	HPSE_HUMAN
String	9606.ENSP00000384262
Molecular Weight	Calculated MW: 17.6 kDa Observed MW (SDS-PAGE): 20 kDa
Sequence Fragment	Pro400-Ile543
Sequence	PLPDYWLSLL FKKLVGTKVL MASVQGSKRR KLRVYLHCTN TDNPRYKEGD LTLYAINLHN VTKYLRLPYP FSNKQVDKYL LRPLGPHGLL SKSVQLNGLT LKMVDDQTLP PLMEKPLRPG SSLGLPAFSY SFFVIRNAKV AACI
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.05% Sarcosyl and 5% Trehalose.
Activity	Active
Biological Activity	Heparanase (HPA), also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Epithelial Cell Transforming Sequence 2 (ECT2) has been identified as an interactor of HPSE, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HPSE and recombinant human ECT2. Briefly, HPSE was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to ECT2-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-HPSE polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of HPSE and ECT2 is shown in Figure 3.
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Human Heparanase (HPA) Protein (Active)

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