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Human Hepcidin (HAMP) Protein (Active)
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Catalogue No: abx655669
Price:
US$435.00
(Size: 50 µg)
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Human Hepcidin (HAMP) Protein (Active) is an Active Recombinant Human protein expressed in E. coli.
Research Articles on Hepcidin (HAMP)
| Target | Hepcidin (HAMP) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cell |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P81172 (UniProt, ExPASy) |
| UniProt Secondary AC | Q1HE14, Q9BY68 |
| UniProt Entry Name | HEPC_HUMAN |
| Gene Symbol | HAMP |
| GeneID | 57817 |
| KEGG | hsa:57817 |
| String | 9606.ENSP00000471894 |
| Molecular Weight | Calculated MW: 34.1 kDa Observed MW (SDS-PAGE): 34 kDa |
| Sequence Fragment | Ser25-Thr84 |
| Sequence | SVFPQQ TGQLAELQPQ DRAGARASWM PMFQRRRRRD THFPICIFCC GCCHRSKCGM CCKT |
| Tag | N-terminal His Tag and Fc tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose. |
| Activity | Active |
| Biological Activity | Hepcidin is a regulator of iron metabolism, inhibiting iron transport by binding to the iron export channel Ferroportin (FPN) which is located on the basolateral surface of gut enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepcidin ultimately breaks down the transporter protein in the lysosome. Inhibiting FPN prevents iron from being exported, resulting in iron being sequestered in the cells. A binding ELISA assay was conducted to detect the interaction of recombinant human Hepcidin and recombinant human FPN. Briefly, Hepcidin was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FPN-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-Hepcidin polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of Hepcidin and FPN is shown in Figure 4. |
| Endotoxin Level | < 1.0 EU/µg (LAL method) |
| Concentration | Prior to lyophilization: 400 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |