Human Hepcidin (HAMP) Protein (Active)

Human Hepcidin (HAMP) Protein (Active) is an Active Recombinant Human protein expressed in E. coli.

Target	Hepcidin (HAMP)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	293F cell
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P81172 (UniProt, ExPASy)
UniProt Secondary AC	Q1HE14, Q9BY68
UniProt Entry Name	HEPC_HUMAN
Gene Symbol	HAMP
GeneID	57817
KEGG	hsa:57817
String	9606.ENSP00000471894
Molecular Weight	Calculated MW: 34.1 kDa Observed MW (SDS-PAGE): 34 kDa
Sequence Fragment	Ser25-Thr84
Sequence	SVFPQQ TGQLAELQPQ DRAGARASWM PMFQRRRRRD THFPICIFCC GCCHRSKCGM CCKT
Tag	N-terminal His Tag and Fc tag
Buffer	Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose.
Activity	Active
Biological Activity	Hepcidin is a regulator of iron metabolism, inhibiting iron transport by binding to the iron export channel Ferroportin (FPN) which is located on the basolateral surface of gut enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepcidin ultimately breaks down the transporter protein in the lysosome. Inhibiting FPN prevents iron from being exported, resulting in iron being sequestered in the cells. A binding ELISA assay was conducted to detect the interaction of recombinant human Hepcidin and recombinant human FPN. Briefly, Hepcidin was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FPN-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-Hepcidin polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of Hepcidin and FPN is shown in Figure 4.
Endotoxin Level	< 1.0 EU/µg (LAL method)
Concentration	Prior to lyophilization: 400 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Human Hepcidin (HAMP) Protein (Active)

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