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Human Interferon Beta (IFNb) Protein (Active)
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Catalogue No: abx655691
Price:
US$565.50
(Size: 50 µg)
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Human Interferon Beta (IFNb) Protein (Active) is an Active Recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Interferon Beta (IFNb)
| Target | Interferon Beta (IFNb) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 98% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P01574 (UniProt, ExPASy) |
| UniProt Secondary AC | Q5VWC9 |
| UniProt Entry Name | IFNB_HUMAN |
| Gene Symbol | IFNB |
| GeneID | 3456 |
| KEGG | hsa:3456 |
| String | 9606.ENSP00000369581 |
| Molecular Weight | Calculated MW: 23.7 kDa Observed MW (SDS-PAGE): 25 kDa |
| Sequence Fragment | Met22-Asn187 |
| Sequence | MSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR LTGYLRN |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | A proliferation assay was conducted to detect the bioactivity of recombinant human IFNb using A549 cells. Briefly, A549 cells were seeded into triplicate wells in 96-well plates at a density of 2,000 cells/well and allowed to attach with serum-free medium overnight, then the medium was replaced with 5% serum standard DMEM prior to the addition of various concentrations of IFNb. After incubation for 96 h, cells were observed by inverted microscope (Figure 3) and cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay (Figure 4). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C. The ED50 of recombinant human IFNb is 6.4 µg/ml. |
| Endotoxin Level | < 1.0 EU per 1 μg (LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |