+44 (0) 1223 755950
+1 832 327 7413
Click on the image to see the image legend
Target | Laminin Subunit Alpha 3 (LAMA3) |
Origin | Human |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 90% |
Reconstitution | Reconstitute in ddH2O. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | Q16787 (UniProt, ExPASy) |
UniProt Secondary AC | B0YJ33, Q13679, Q13680, Q6VU67, Q6VU68, Q6VU69, Q76E14, Q96TG0 |
UniProt Entry Name | LAMA3_HUMAN |
Gene Symbol | LAMA3 |
GeneID | 3909 |
OMIM | 226700 |
HGNC | 6483 |
KEGG | hsa:3909 |
Ensembl | ENSG00000053747 |
String | 9606.ENSP00000324532 |
Molecular Weight | Calculated MW: 28.3 kDa Observed MW (SDS-PAGE): 33 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
Sequence Fragment | Gln47-Leu296 |
Sequence | QASY VEFRPSQGCS PGYYRDHKGL YTGRCVPCNC NGHSNQCQDG SGICVNCQHN TAGEHCERCQ EGYYGNAVHG SCRACPCPHT NSFATGCVVN GGDVRCSCKA GYTGTQCERC APGYFGNPQK FGGSCQPCSC NSNGQLGSCH PLTGDCINQE PKDSSPAEEC DDCDSCVMTL LNDLATMGEQ LRLVKSQLQG LSASAGLLEQ MRHMETQAKD LRNQLLNYRS AISNHGSKIE GLERELTDLN QEFETL |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
Activity | Not tested |
Concentration | Prior to lyophilization: 200 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |