Human C-C Motif Chemokine 2 / MCP1 (CCL2) Protein

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Catalogue No: abx068048
Price: US$246.50
(Size: 10 µg)

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Datasheet SDS
Recombinant C-C Motif Chemokine 2 / MCP1 (CCL2) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).

Target C-C Motif Chemokine 2 / MCP1 (CCL2)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P13500 (UniProt, ExPASy)
UniProt Secondary AC B2R4V3, Q9UDF3
UniProt Entry Name CCL2_HUMAN
Gene Symbol CCL2
GeneID 6347
OMIM 158105
NCBI Accession NP_002973.1 NM_002982.3
HGNC 10618
KEGG hsa:6347
Ensembl ENSG00000108691
String 9606.ENSP00000225831
Molecular Weight Calculated MW: 9.5 kDa
Observed MW (SDS-PAGE): 15 kDa, 17 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Gln24-Thr99
Sequence QPDAINA PVTCCYNFTN RKISVQRLAS YRRITSSKCP KEAVIFKTIV AKEICADPKQ KWVQDSMDHL DKQTQTPKT
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on C-C Motif Chemokine 2 / MCP1 (CCL2)


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