+44 (0) 1223 755950
+1 832 327 7413
Human Neutrophil Elastase / ELA2 (ELANE) Protein (Active)
REQUEST MORE INFO
Catalogue No: abx651290
Price:
US$536.50
(Size: 50 µg)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
Human Neutrophil Elastase is a recombinant active Human protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Neutrophil Elastase / ELA2 (ELANE)
| Target | Neutrophil Elastase / ELA2 (ELANE) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 98% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P08246 (UniProt, ExPASy) |
| UniProt Secondary AC | P09649, Q6B0D9, Q6LDP5 |
| UniProt Entry Name | ELNE_HUMAN |
| KEGG | hsa:1991 |
| String | 9606.ENSP00000466090 |
| Molecular Weight | Calculated MW: 53.3 kDa Observed MW: 53 kDa (SDS-PAGE) |
| Sequence Fragment | Ile30-Gln247 |
| Sequence | I VGGRRARPHA WPFMVSLQLR GGHFCGATLI APNFVMSAAH CVANVNVRAV RVVLGAHNLS RREPTRQVFA VQRIFENGYD PVNLLNDIVI LQLNGSATIN ANVQVAQLPA QGRRLGNGVQ CLAMGWGLLG RNRGIASVLQ ELNVTVVTSL CRRSNVCTLV RGRQAGVCFG DSGSPLVCNG LIHGIASFVR GGCASGLYPD AFAPVAQFVN WIDSIIQ |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, 0.01% sarcosyl and 5% trehalose. |
| Activity | Active |
| Biological Activity | Collagen Type XVII (COL17) has been known to interact with Neutrophil Elastase (NE/ELA2). A binding ELISA assay was conducted to determine the interaction of recombinant human COL17 and recombinant human NE/ELA2. Briefly, ELA2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to COL17-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-ELA2 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of NE/ELA2 and COL17 is shown in Figure 2. |
| Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
| Concentration | Prior to lyophilization: 80 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |