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Human Pyruvate Dehydrogenase Alpha (PDHa) Protein
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Catalogue No: abx068801
Price:
US$261.00
(Size: 10 µg)
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Recombinant Pyruvate Dehydrogenase Alpha (PDHa) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Pyruvate Dehydrogenase Alpha (PDHa)
| Target | Pyruvate Dehydrogenase Alpha (PDHa) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | Reconstitute in dH2O. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P08559 (UniProt, ExPASy) |
| UniProt Entry Name | ODPA_HUMAN |
| KEGG | hsa:5160 |
| String | 9606.ENSP00000369134 |
| Molecular Weight | Calculated MW: 41.5 kDa Observed MW (SDS-PAGE): 46 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Phe30-Ser390 |
| Sequence | F ANDATFEIKK CDLHRLEEGP PVTTVLTRED GLKYYRMMQT VRRMELKADQ LYKQKIIRGF CHLCDGQEAC CVGLEAGINP TDHLITAYRA HGFTFTRGLS VREILAELTG RKGGCAKGKG GSMHMYAKNF YGGNGIVGAQ VPLGAGIALA CKYNGKDEVC LTLYGDGAAN QGQIFEAYNM AALWKLPCIF ICENNRYGMG TSVERAAAST DYYKRGDFIP GLRVDGMDIL CVREATRFAA AYCRSGKGPI LMELQTYRYH GHSMSDPGVS YRTREEIQEV RSKSDPIMLL KDRMVNSNLA SVEELKEIDV EVRKEIEDAA QFATADPEPP LEELGYHIYS SDPPFEVRGA NQWIKFKSVS |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 100mM NaHCO3, 500mM NaCl, pH 8.3, containing 0.01% Sarcosyl and 5% Trehalose. |
| Activity | Not tested |
| Concentration | Prior to lyophilization: 1000 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |