+44 (0) 1223 755950
+1 832 327 7413
Human Peptidylglycine Alpha Amidating Monooxygenase (PAM) Protein (Active)
REQUEST MORE INFO
Catalogue No: abx655796
Price:
US$768.50
(Size: 50 µg)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
Human Peptidylglycine Alpha Amidating Monooxygenase (PAM) Protein (Active) is an Active Recombinant Human protein.
Research Articles on Peptidylglycine Alpha Amidating Monooxygenase (PAM)
| Target | Peptidylglycine Alpha Amidating Monooxygenase (PAM) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P19021 (UniProt, ExPASy) |
| UniProt Entry Name | AMD_HUMAN |
| Gene Symbol | PAM |
| Molecular Weight | Calculated MW: 33.7 kDa Observed MW (SDS-PAGE): 34 kDa |
| Sequence Fragment | Phe21-Cys288 |
| Sequence | FRSPLSVFKR FKETTRPFSN ECLGTTRPVV PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARC |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Human Peptidylglycine Alpha Amidating Monooxygenase (PAM) Protein is a enzyme required for biosynthesis of many signalling peptides. The catalytic domains of PAM catalyse neuroendocrine peptides to active alpha-amidated products. The binding activity of recombinant PAM with recombinant Glucosidase Alpha, Acid (GaA) was determined in a binding ELISA. Briefly, PAM was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to GaA-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-PAM polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of PAM and GaA is shown in Figure 4. |
| Endotoxin Level | < 1.0 EU/µg (LAL method) |
| Concentration | Prior to lyophilization: 100 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |