Human Protein S100-A8 / CAGA (S100A8) Protein (Active)

Human S100 Calcium Binding Protein A8 (S100A8) Protein (Active) is an Active Recombinant Human protein.

Target	Protein S100-A8 / CAGA (S100A8)
Origin	Human
Expression	Recombinant
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 95%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 12.1 kDa Observed MW (SDS-PAGE): 12 kDa
Sequence Fragment	Met1-Glu93
Sequence	MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE
Tag	N-terminal His tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity	Active
Biological Activity	Human S100 Calcium Binding Protein A8 (S100A8), also known as Calgranulin A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localised in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Human S100 Calcium Binding Protein A9 (S100A9) has been identified as an interactor of S100A8, thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100A8 and recombinant human S100A9. Briefly, S100A8 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to S100A9-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-S100A8 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of S100A8 and S100A9 is shown in Figure 4.
Endotoxin Level	< 1.0 EU per 1 µg
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

Research Articles on Protein S100-A8 / CAGA (S100A8)

Human Protein S100-A8 / CAGA (S100A8) Protein (Active)

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