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Human Sphingomyelin Phosphodiesterase (SMPD1) Protein (Active)
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Catalogue No: abx655956
Price:
US$768.50
(Size: 50 µg)
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Human SMPD1 is an active recombinant Human protein produced in a Eukaryotic expression system (293F cell).
Research Articles on Sphingomyelin Phosphodiesterase (SMPD1)
| Target | Sphingomyelin Phosphodiesterase (SMPD1) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cell |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.6. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.6, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P17405 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K8M3, E9PKS3, P17406, Q13811, Q16837, Q16841 |
| UniProt Entry Name | ASM_HUMAN |
| Gene Symbol | SMPD1 |
| GeneID | 6609 |
| OMIM | 607608 |
| HGNC | 11120 |
| KEGG | hsa:6609 |
| Ensembl | ENSG00000166311 |
| String | 9606.ENSP00000340409 |
| Molecular Weight | Calculated MW: 64.9 kDa Observed MW (SDS-PAGE): 75 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | His62-Pro628 |
| Sequence | HPLSPQGHP ARLHRIVPRL RDVFGWGNLT CPICKGLFTA INLGLKKEPN VARVGSVAIK LCNLLKIAPP AVCQSIVHLF EDDMVEVWRR SVLSPSEACG LLLGSTCGHW DIFSSWNISL PTVPKPPPKP PSPPAPGAPV SRILFLTDLH WDHDYLEGTD PDCADPLCCR RGSGLPPASR PGAGYWGEYS KCDLPLRTLE SLLSGLGPAG PFDMVYWTGD IPAHDVWHQT RQDQLRALTT VTALVRKFLG PVPVYPAVGN HESTPVNSFP PPFIEGNHSS RWLYEAMAKA WEPWLPAEAL RTLRIGGFYA LSPYPGLRLI SLNMNFCSRE NFWLLINSTD PAGQLQWLVG ELQAAEDRGD KVHIIGHIPP GHCLKSWSWN YYRIVARYEN TLAAQFFGHT HVDEFEVFYD EETLSRPLAV AFLAPSATTY IGLNPGYRVY QIDGNYSGSS HVVLDHETYI LNLTQANIPG AIPHWQLLYR ARETYGLPNT LPTAWHNLVY RMRGDMQLFQ TFWFLYHKGH PPSEPCGTPC RLATLCAQLS ARADSPALCR HLMPDGSLPE AQSLWPRP |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose. |
| Activity | Active |
| Biological Activity | Specific activity: > 1000 pmol/min/µg |
| Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |