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Human T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein (Active)
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Catalogue No: abx655966
Price:
US$478.50
(Size: 50 µg)
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Human T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein is a recombinant active Human protein.
Research Articles on T-lymphocyte activation antigen CD80 / B7-1 (Anti-CD80)
| Target | T-lymphocyte activation antigen CD80 / B7-1 (Anti-CD80) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | ELISA |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P33681 (UniProt, ExPASy) |
| Molecular Weight | Calculated MW: 25.5kDa Observed MW (SDS-PAGE): 40-60 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Val35-Asn242 |
| Sequence | VIHVTK EVKEVATLSC GHNVSVEELA QTRIYWQKEK KMVLTMMSGD MNIWPEYKNR TIFDITNNLS IVILALRPSD EGTYECVVLK YEKDAFKREH LAEVTLSVKA DFPTPSISDF EIPTSNIRRI ICSTSGGFPE PHLSWLENGE ELNAINTTVS QDPETELYAV SSKLDFNMTT NHSFMCLIKY GHLRVNQTFN WNTTKQEHFP DN |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose. |
| Activity | Active |
| Biological Activity | EC50: 0.412 µg/ml A functional binding ELISA assay was conducted to detect the interaction of recombinant Human CD80 with recombinant human CD86. Briefly, serial dilutions of recombinant rat Human T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein were carried out in PBS, 0.01 % BSA (pH 7.4). Duplicate samples of 100 µl were transferred to microtiter wells pre-coated with CD86 and incubated for 1h at 37 °C. Wells were washed with PBST 3 times and incubated with Streptavidin-HRP for 40 minutes, then aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 °C. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant Human CD80 and recombinant human CD86 was shown in Figure 1. |
| Endotoxin Level | < 1.0 EU/µg (determined by the LAL method) |
| Concentration | Prior to lyophilization: 1100 µg/ml |
| Availability | Shipped within 5-12 working days. |
| Note | This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |