Human Tyrosine Aminotransferase (TAT) Protein (Active)

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Catalogue No: abx655855
Price: US$391.50
(Size: 50 µg)
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SDS-PAGE analysis of recombinant Human TAT Protein.
Human Tyrosine Aminotransferase (TAT) Protein (Active) Human Tyrosine Aminotransferase (TAT) Protein (Active) Human Tyrosine Aminotransferase (TAT) Protein (Active)

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Datasheet SDS
Human Tyrosine Aminotransferase (TAT) Protein (Active) is an Active Recombinant Human protein.

Target Tyrosine Aminotransferase (TAT)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Conjugation Unconjugated
Form Lyophilized
Purity > 96%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P17735 (UniProt, ExPASy)
UniProt Secondary AC B2R8I1, D3DWS2
UniProt Entry Name ATTY_HUMAN
Gene Symbol TAT
GeneID 6898
KEGG hsa:6898
String 9606.ENSP00000348234
Molecular Weight Calculated MW: 30.0 kDa
Observed MW (SDS-PAGE): 33 kDa
Sequence Fragment Cys221-Lys454
Sequence CGSVFSKRHL QKILAVAARQ CVPILADEIY GDMVFSDCKY EPLATLSTDV PILSCGGLAK RWLVPGWRLG WILIHDRRDI FGNEIRDGLV KLSQRILGPC TIVQGALKSI LCRTPGEFYH NTLSFLKSNA DLCYGALAAI PGLRPVRPSG AMYLMVGIEM EHFPEFENDV EFTERLVAEQ SVHCLPATCF EYPNFIRVVI TVPEVMMLEA CSRIQEFCEQ HYHCAEGSQE ECDK
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Active
Biological Activity Tyrosine aminotransferase (TAT) is an enzyme that catalyzes the conversion of tyrosine to 4-hydroxyphenylpyruvate. Heat Shock 70 kDa Protein 8 (HSPA8) has been identified as an interactor of TAT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TAT and recombinant human HSPA8. Briefly, TAT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSPA8-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-TAT polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of TAT and HSPA8 is shown in Figure 3.
Endotoxin Level < 1.0 EU/µg (LAL method)
Concentration Prior to lyophilization: 400 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Tyrosine Aminotransferase (TAT)
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