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Target | Tyrosine Aminotransferase (TAT) |
Origin | Human |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 96% |
Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P17735 (UniProt, ExPASy) |
UniProt Secondary AC | B2R8I1, D3DWS2 |
UniProt Entry Name | ATTY_HUMAN |
Gene Symbol | TAT |
GeneID | 6898 |
KEGG | hsa:6898 |
String | 9606.ENSP00000348234 |
Molecular Weight | Calculated MW: 30.0 kDa Observed MW (SDS-PAGE): 33 kDa |
Sequence Fragment | Cys221-Lys454 |
Sequence | CGSVFSKRHL QKILAVAARQ CVPILADEIY GDMVFSDCKY EPLATLSTDV PILSCGGLAK RWLVPGWRLG WILIHDRRDI FGNEIRDGLV KLSQRILGPC TIVQGALKSI LCRTPGEFYH NTLSFLKSNA DLCYGALAAI PGLRPVRPSG AMYLMVGIEM EHFPEFENDV EFTERLVAEQ SVHCLPATCF EYPNFIRVVI TVPEVMMLEA CSRIQEFCEQ HYHCAEGSQE ECDK |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Active |
Biological Activity | Tyrosine aminotransferase (TAT) is an enzyme that catalyzes the conversion of tyrosine to 4-hydroxyphenylpyruvate. Heat Shock 70 kDa Protein 8 (HSPA8) has been identified as an interactor of TAT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TAT and recombinant human HSPA8. Briefly, TAT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HSPA8-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-TAT polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of TAT and HSPA8 is shown in Figure 3. |
Endotoxin Level | < 1.0 EU/µg (LAL method) |
Concentration | Prior to lyophilization: 400 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |