+44 (0) 1223 755950
+1 832 327 7413
Hypoxia Inducible Factor 1 Alpha (HIF1A) Antibody
REQUEST MORE INFO
Catalogue No: abx239939
Price:
US$406.00
(Size: 100 µg)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
Hypoxia Inducible Factor 1 Alpha (HIF1A) Antibody is a Mouse Monoclonal antibody for the detection of HIF1a. Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256,.PMID: 7539918).
Research Articles on Hypoxia Inducible Factor 1 Alpha (HIF1A)
| Target | Hypoxia Inducible Factor 1 Alpha (HIF1A) |
| Clonality | Monoclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IF/ICC |
| Host | Mouse |
| Recommended dilutions | WB: 1/1000 - 1/10000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) |
| Isotype | IgG1 |
| Form | Liquid |
| Purity | ≥ 95% (SDS-PAGE) |
| Purification | Purified by Protein A and Protein G affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Validity | 12 months. |
| UniProt Primary AC | Q16665 (UniProt, ExPASy) |
| UniProt Secondary AC | C0LZJ3, Q53XP6, Q96PT9, Q9UPB1 |
| Gene Symbol | HIF1A |
| GeneID | 3091 |
| OMIM | 603348 |
| HGNC | 4910 |
| Ensembl | ENSG00000100644 |
| Molecular Weight | Observed MW: 120 kDa |
| Buffer | PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol. |
| Concentration | 2 mg/ml |
| Availability | Shipped within 5-12 working days. |
| Note | This product is for research use only. |