+44 (0) 1223 755950
+1 832 327 7413
Hypoxia Inducible Factor 1 Alpha (HIF1A) Antibody
REQUEST MORE INFO
Catalogue No: abx240089
Price:
US$406.00
(Size: 100 µg)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
Hypoxia Inducible Factor 1 Alpha (HIF1A) Antibody is a Rabbit Polyclonal antibody for the detection of HIF1a. Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.
Research Articles on Hypoxia Inducible Factor 1 Alpha (HIF1A)
| Target | Hypoxia Inducible Factor 1 Alpha (HIF1A) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500 - 1/2000, IHC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor) |
| Isotype | IgG |
| Form | Liquid |
| Purity | ≥ 95% (SDS-PAGE) |
| Purification | Purified by immunogen affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Validity | 12 months. |
| UniProt Primary AC | Q16665 (UniProt, ExPASy) |
| UniProt Secondary AC | C0LZJ3, Q53XP6, Q96PT9, Q9UPB1 |
| Gene Symbol | HIF1A |
| GeneID | 3091 |
| OMIM | 603348 |
| HGNC | 4910 |
| Ensembl | ENSG00000100644 |
| Molecular Weight | Observed MW: 120 kDa |
| Buffer | PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol. |
| Concentration | 2 mg/ml |
| Availability | Shipped within 5-12 working days. |
| Note | This product is for research use only. |