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Target | Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Alpha Phospho-Thr23 (CHUK pT23) |
Clonality | Polyclonal |
Target Modification | Thr23 |
Modification | Phosphorylation |
Reactivity | Human, Mouse, Rat |
Tested Applications | ELISA, WB, IHC |
Host | Rabbit |
Recommended dilutions | ELISA: 1/5000, WB: 1/500 - 1/3000, IHC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | Synthesized phosphopeptide derived from human CHUK/IKKA around the phosphorylation site of Threonine 23 (L-G-TP-G-G). |
Isotype | IgG |
Form | Liquid |
Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | O15111 (UniProt, ExPASy) |
UniProt Secondary AC | O14666, Q13132, Q5W0I4, Q92467 |
UniProt Entry Name | IKKA_HUMAN |
Gene Symbol | CHUK |
GeneID | 1147 |
OMIM | 600664 |
HGNC | 1974 |
KEGG | hsa:1147 |
Ensembl | ENSG00000213341 |
String | 9606.ENSP00000359424 |
Sequence | CMRERLGTGGFGNV |
Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
Specificity | Detects endogenous levels of CHUK/IKKA only when phosphorylated at Threonine 23. |
Concentration | 1 mg/ml |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |