DNA-binding protein Ikaros (IKZF1) Antibody is a Rabbit Polyclonal antibody against DNA-binding protein Ikaros (IKZF1). This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).
Target |
DNA-Binding Protein Ikaros (IKZF1) |
Clonality |
Polyclonal |
Reactivity |
Human, Mouse, Rat |
Tested Applications |
WB, IF/ICC |
Host |
Rabbit |
Recommended dilutions |
WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200, ChIP: 5 µg/10-15 µg Chromatin. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Ikaros. |
Isotype |
IgG |
Form |
Liquid |
Purification |
Purified by affinity chromatography. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC |
Q13422 (UniProt,
ExPASy)
|
UniProt Secondary AC |
A4D260, B4E0Z1, D3DVM5, O00598, Q53XL2, Q69BM4, Q8WVA3 |
UniProt Entry Name |
IKZF1_HUMAN |
Gene Symbol |
IKZF1 |
GeneID |
10320
|
NCBI Accession |
NP_001207694.1
|
KEGG |
hsa:10320
|
String |
9606.ENSP00000331614
|
Molecular Weight |
Calculated MW: 58 kDa Observed MW: 50-70 kDa |
Buffer |
PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
Concentration |
> 0.2 mg/ml |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Research Articles on DNA-Binding Protein Ikaros (IKZF1)