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DNA-Binding Protein Ikaros (IKZF1) Antibody
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Catalogue No: abx001530
Price:
US$217.50
(Size: 20 µl)
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DNA-binding protein Ikaros (IKZF1) Antibody is a Rabbit Polyclonal antibody against DNA-binding protein Ikaros (IKZF1). This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL).
Research Articles on DNA-Binding Protein Ikaros (IKZF1)
| Target | DNA-Binding Protein Ikaros (IKZF1) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF/ICC, ChIP |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200, ChIP: 5 µg antibody per 10 µg - 15 µg of Chromatin. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-270 of human Ikaros. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q13422 (UniProt, ExPASy) |
| UniProt Secondary AC | A4D260, B4E0Z1, D3DVM5, O00598, Q53XL2, Q69BM4, Q8WVA3 |
| UniProt Entry Name | IKZF1_HUMAN |
| Gene Symbol | IKZF1 |
| GeneID | 10320 |
| NCBI Accession | NP_001207694.1 |
| KEGG | hsa:10320 |
| String | 9606.ENSP00000331614 |
| Molecular Weight | Calculated MW: 58 kDa Observed MW: 50-70 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |