Interleukin-27 Subunit Alpha (IL27) Antibody

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Catalogue No: abx004213
Price: US$797.50
(Size: 96 tests)
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Western blot analysis of various lysates using IL27 Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Interleukin-27 Subunit Alpha (IL27) Antibody

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Datasheet SDS
IL27 Antibody is a Rabbit Polyclonal antibody against IL27. The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Target Interleukin-27 Subunit Alpha (IL27)
Clonality Polyclonal
Reactivity Human, Mouse
Tested Applications ELISA, WB
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-243 of human IL27.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q8NEV9 (UniProt, ExPASy)
UniProt Secondary AC A0N0L2, Q6P676
UniProt Entry Name IL27A_HUMAN
Gene Symbol IL27
GeneID 246778
NCBI Accession NP_663634.2
KEGG hsa:246778
String 9606.ENSP00000349365
Molecular Weight Calculated MW: 27 kDa
Observed MW: 55 kDa
Buffer PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Interleukin-27 Subunit Alpha (IL27)
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