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Target | Immunoglobulin G1 (IgG1) |
Origin | Human |
Expression | Native |
Tested Applications | WB, SDS-PAGE |
Host | Human |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 90% |
Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Molecular Weight | Calculated MW: 146.0 kDa Observed MW: 55 kDa, 22 kDa As a result of the disulfide bonds in human IgG1, two lines are observed at approximately 55kDa, which corresponds to the heavy chain, and two lines are observed at approximately 25kDa, which corresponds to the light chain, in SDS-PAGE under reducing conditions. |
Sequence | This product is a native protein and the sequence has not been determined. |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
Activity | Not tested |
Concentration | Prior to lyophilization: 200 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |