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Antibody Pair Support Kit (Sandwich Method)
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Catalogue No: abx098958
Price:
US$261.00
(Size: 10 × 96 tests)
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Datasheet SDS
Antibody Pair Support Kit (Sandwich Method) contains reagents for use with an antibody pair for a Sandwich ELISA or CLIA. Kit contents:
- Coating Buffer: 1 vial, lyophilized powder
- Blocking Buffer: 1 vial, lyophilized powder
- Standard Diluent: 1 vial, lyophilized powder
- Detection Antibody Diluent: 1 vial, lyophilized powder
- Streptavidin-HRP Diluent: 1 vial, lyophilized powder
- Wash Buffer: 2 vials, lyophilized powder
- Streptavidin-HRP: 1 vial, 1.2 ml
- TMB Substrate Solution: 2 vials, 48 ml each
- Stop Solution: 2 vials, 30 ml each
- Coating Buffer: Reconstitute in 220 ml distilled water.
- Blocking Buffer: Reconstitute in 240 ml distilled water.
- Standard Diluent: Reconstitute in 160 ml distilled water.
- Detection Antibody Diluent: Reconstitute in 120 ml distilled water.
- Streptavidin-HRP Diluent: Reconstitute in 120 ml distilled water.
- Wash Buffer: Reconstitute 1 vial in 2000 ml distilled water.
| Reactivity | General (All species) |
| Tested Applications | ELISA |
| Recommended dilutions | Streptavidin-HRP should be diluted 1/100 using Streptavidin-HRP Diluent. |
| Reconstitution | Reconstitute and prepare working solutions before carrying out the assay. All working solutions should be colourless. |
| Storage | Store Streptavidin-HRP at -20 °C. All other components can be stored at 4 °C for up to 6 months. Reconstituted and working (appropriately diluted) solutions should be used immediately but can be stored at 4 °C for up to one week. Avoid repeated freeze/thaw cycles. It is recommended that solutions are made fresh before the experiment. |
| Buffer | The Stop Solution contains < 10% sulphuric acid. |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. |
| Directions for use | Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. |