ELISA Development Support Kit (Sandwich Method)
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Price:
US$333.50
(Size: 5 × 96 tests)
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ELISA Development Support Kit (Sandwich Method) contains reagents for use with an ELISA Development Kit for a Sandwich ELISA.
Kit contents: - Coating Buffer (5X): 14 ml
- Blocking Buffer: 100 ml
- Standard and Sample Diluent: 60 ml
- Detection Antibody Diluent: 60 ml
- HRP-Conjugate Diluent: 60 ml
- Wash Buffer (25X): 55 ml
- TMB Substrate: 50 ml
- Stop Solution (5X): 5 ml
- Plate Sealer: 25
Target |
ELISA Development Support Kit (Sandwich Method) |
Reactivity |
General (All species) |
Tested Applications |
ELISA |
Recommended dilutions |
Prepare working solutions before carrying out the assay. - Coating Buffer: Dilute to 1X with double-distilled water.
- Wash Buffer: Dilute to 1X with double-distilled water. If crystals are observed in the 25X concentrated soluton, warm to 40 °C in a water bath to dissolve the crystals before carrying out the dilution.
- Stop Solution: Dilute to 1X with double-distilled water.
The Blocking Buffer, Standard and Sample Diluent, Detection Antibody Diluent, HRP-Conjugate Diluent and TMB Substrate are ready-to-use and do not require further dilution. |
Storage |
Store between 2°- 8°C. |
Validity |
12 months. |
Buffer |
- Coating Buffer: 1X Citrate-Buffered Saline
- Blocking Buffer: 1X PBS containing stabilizing proteins
- Wash Buffer: 3% Tris
- Standard and Sample Diluent Buffer: 1X PBS containing stabilizing proteins
- Detection Antibody Diluent: 1X PBS containing stabilizing proteins
- HRP-Conjugate Diluent: 1X PBS containing stabilizing proteins
- Stop Solution: 5% Sulfuric Acid
|
Availability |
Shipped within 5-7 working days. |
Note |
This product is for research use only. |
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