Human Methyl CpG Binding Protein 2 (MECP2) Protein

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Catalogue No: abx650076
Price: US$304.50
(Size: 10 µg)

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Datasheet SDS
Human MECP2 Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).

This protein is the immunogen for the following antibodies: abx131551, abx173560

Target Methyl CpG Binding Protein 2 (MECP2)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P51608 (UniProt, ExPASy)
UniProt Secondary AC O15233, Q6QHH9, Q7Z384
UniProt Entry Name MECP2_HUMAN
Gene Symbol MECP2
GeneID 4204
HGNC 690
KEGG hsa:4204
String 9606.ENSP00000395535
Molecular Weight Calculated MW: 23.4 kDa
Observed MW (SDS-PAGE): 30 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Met1-Lys177
Sequence MVAGMLGLRE EKSEDQDLQG LKDKPLKFKK VKKDKKEEKE GKHEPVQPSA HHSAEPAEAG KAETSEGSGS APAVPEASAS PKQRRSIIRD RGPMYDDPTL PEGWTRKLKQ RKSGRSAGKY DVYLINPQGK AFRSKVELIA YFEKVGDTSL DPNDFDFTVT GRGSPSRREQ KPPKKPK
Tag N-terminal His tag
Buffer Prior to lyophilization: 100 mM NaHCO3, 500 mM NaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Methyl CpG Binding Protein 2 (MECP2)


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