+44 (0) 1223 755950
+1 832 327 7413
Human Interleukin 17C (IL17C) Protein
REQUEST MORE INFO
Catalogue No: abx651383
Price:
US$536.50
(Size: 50 µg)
Reviews: 
(0 Publications and Reviews)
(0 Publications and Reviews)
Click on the image to see the image legend
Interleukin 17C (IL17C) Protein (Active) is an active Human protein expressed in E. coli
Research Articles on Interleukin 17C (IL17C)
| Target | Interleukin 17C (IL17C) |
| Origin | Human |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q9P0M4 (UniProt, ExPASy) |
| UniProt Secondary AC | Q3MIG8, Q9HC75 |
| UniProt Entry Name | IL17C_HUMAN |
| String | 9606.ENSP00000244241 |
| Molecular Weight | Calculated MW: 21.0 kDa Observed MW (SDS-PAGE): 25 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | MG + His19-Val197 + LEHHHHHH |
| Sequence | MG-HH DPSLRGHPHS HGTPHCYSAE ELPLGQAPPH LLARGAKWGQ ALPVALVSSL EAASHRGRHE RPSATTQCPV LRPEEVLEAD THQRSISPWR YRVDTDEDRY PQKLAFAECL CRGCIDARTG RETAALNSVR LLQSLLVLRR RPCSRDGSGL PTPGAFAFHT EFIHVPVGCT CVLPRSV-LEHHHHHH |
| Tag | C-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Interleukin 17C (IL17C) is a T cell-derived cytokine which has high sequence homology with Interleukin 17 (IL17) and may play a crucial role in innate immunity of the epithelium. IL17C binds to Interleukin 17 receptor A (IL17RA), thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL17C and recombinant human IL17RA. Briefly, IL17C was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to IL17RA-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-IL17C polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of IL17C and IL17RA is shown in Figure 3. |
| Concentration | Prior to lyophilization: 100 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |