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IRAK1 (pS376) Antibody
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Catalogue No: abx031872
Price:
US$326.25
(Size: 80 µl)
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This product is currently in development. The lead time for this product may be several months. Please contact us at [email protected] for an updated lead time before purchasing this product. This product is currently in development. The lead time for this product may be several months. Please contact us at [email protected] for an updated lead time before purchasing this product. IRAK1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. This protein is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.
Research Articles on IRAK1 (pS376)
| Target | IRAK1 (pS376) |
| Clonality | Polyclonal |
| Target Modification | Ser376 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, IF/ICC, DB |
| Host | Rabbit |
| Recommended dilutions | IF/ICC: 1/10 - 1/50, DB: 1/500. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S376 of human IRAK1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P51617 (UniProt, ExPASy) |
| UniProt Secondary AC | D3DWW3, D3DWW4, Q7Z5V4, Q96C06, Q96RL2 |
| UniProt Entry Name | IRAK1_HUMAN |
| NCBI Accession | NP_001020413.1 NP_001020414.1 NP_001560.2 |
| KEGG | hsa:3654 |
| String | 9606.ENSP00000358997 |
| Molecular Weight | Calculated MW: 76.5 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Please enquire. |
| Note | This product is for research use only. |